Lopez launched a clothing line in 2003. Named JLO by Jennifer Lopez, the line included different types of clothing for young women, including jeans, T-shirts, coats, belts, purses, and lingerie, a jewelry line, and an accessory line that includes hats, gloves, and scarves. Lopez participated in the Louis Vuitton Winter 2003 campaign. In 2005, she launched a new clothing line called Sweetface. In late 2007, Lopez retired JLO by Jennifer Lopez and launched a new juniors' line called JustSweet. Her fashion lines have featured at many New York Fashion Week events.

Lopez's frequent use of animal fur in her clothing lines and personal wardrobe has brought the scorn of people concerned with animal rights. At the Los Angeles premiere of Monster-in-Law, more than one hundred protesters from People for the Ethical Treatment of Animals (PETA) held a demonstration to highlight their concerns. Lopez told a radio DJ she was open to being educated on the topic. On April 12, 2002, Lopez opened a Cuban restaurant in the South Lake district of Pasadena, California named Madre's. A media sensation was creating when during a press shoot for the fancy restaurant, a mysterious man appeared out of the sidelines and gave Lopez a floral arrangement from Ben Affleck, even as then-husband Cris Judd stood next to her. Lopez was widely criticized for what appeared to be infidelity on her part.

Lopez has ventured in the perfume industry, with her debut "Glow by J.Lo". In October 2003, Lopez introduced a perfume called "Still", having revisit "Glow" the previous year by creating a limited edition spin-off, "Miami Glow by J.Lo", in homage to her adopted hometown of Miami. Lopez also marketed a "Glow" line of body lotions and bronzing products. For the Christmas season of 2005, she launched another fragrance, "Live by Jennifer Lopez". For 2006 Valentine's Day, "Miami Glow" was replaced by yet another "Glow" spin-off, "Love at First Glow by J.Lo". Her following fragrance, "Live Luxe", was released in August 2006, with "Glow After Dark" following in January 2007.

Lopez owns the film and television production company Nuyorican Productions, which she co-founded with her manager, Benny Medina. Dancer and ex-husband Cris Judd once had a stake in the company when he and Lopez were still married. Lopez is a spokesperson for Lux shampoo in Japan, appearing in the product's television commercials. Lopez has been recognized by People en Español magazine as both the cover subject for the "50 Most Beautiful" issue in 2006 and the "100 Most Influential Hispanics" issue in February 2007.

• Plan B (2010) (pre-production)
  
• Cantante, El (2006) .... Puchi
• Bordertown (2006) .... Lauren Adrian
• An Unfinished Life (2005) .... Jean Gilkyson
  ... aka Ein Ungezähmtes Leben (Germany)
• Monster-in-Law (2005) .... Charlotte 'Charlie' Cantilini
  ... aka Das Schwiegermonster (Germany)
• Shall We Dance (2004) .... Paulina
  ... aka Shall We Dance? (USA: poster title)
• Jersey Girl (2004) .... Gertrude Steiney
• Selena: Greatest Hits (2003) (V) .... Selena (segment "Siempre Hace Frio")
• Gigli (2003) .... Ricki
• Maid in Manhattan (2002) .... Marisa Ventura
  ... aka Made in New York (USA: poster title)
• Enough (2002/I) .... Slim Hiller
• Angel Eyes (2001) .... Sharon Pogue
  ... aka Ojos de ángel (USA: Spanish title)
• The Wedding Planner (2001) .... Mary Fiore
  ... aka Wedding Planner - verliebt, verlobt, verplant (Germany)
• The Cell (2000) .... Catherine Deane
  
• Antz (1998) (voice) .... Azteca
• Out of Sight (1998) .... Karen Sisco
• U Turn (1997) .... Grace McKenna
  ... aka U Turn - Ici commence l'enfer (France)
• Anaconda (1997) .... Terri Flores
  ... aka Anaconda (Peru)
• Selena (1997) .... Selena Quintanilla-Pérez
• Blood and Wine (1996) .... Gabriela 'Gabby'
  ... aka Blood & Wine (video box title)
• Jack (1996) .... Miss Marquez