Brooke worked as a model for Venus Swimwear and Frederick's of Hollywood. From 1992 to 2002, Burke became well-known while hosting Wild On!, a popular travel series on the E! Entertainment Television channel, replacing previous host Jules Asner. As part of the series, she traveled the world extensively, profiling many popular travel destinations. Burke first appeared nude during her early modeling career for the website BlueNudes, and most notably after gaining fame in the May 2001 and November 2004 issues of Playboy and has also appeared in lad mags such as Maxim, Stuff, and FHM. In 2007, she was listed as one of Blender's hottest women of film and TV.

Burke has fronted infomercials promoting a Swiss ball video home fitness workout named Gunnar Peterson's Core Secrets, and appears in the first video of the series titled 20 Minute Full Body Workout. The infomercials have screened in New Zealand and Australia. In 2005 and 2006, Burke hosted the CBS summer reality show Rock Star, an American Idol-like series in which aspiring singers compete to become either the frontman or frontwoman for a rock band.

She was the voice and face of "Rachel Teller" in Electronic Arts' video game Need for Speed: Underground 2, and asks the "People and Places" questions in the video game Trivial Pursuit Unhinged. Burke starred as a playable character in the Burger King advergaming title PocketBike Racer and in Big Bumpin'. In 2007, Brooke started a company called Baboosh Baby, which offers wraps for pregnant women. The company's website features a blog from Brooke about her children. Burke, along with Derek Hough as her partner won Season 7 of Dancing with the Stars. Brooke has also starred in the TV show host edition of The Weakest Link.

• "Las Vegas" .... Sheryl (1 episode, 2006)
       - Cash Springs Eternal (2006) TV episode .... Sheryl
• Need for Speed: Underground 2 (2004) (VG) .... Rachel Teller
• "The Bernie Mac Show" (1 episode, 2004)
       - Being Bernie Mac (2004) TV episode
• Knuckle Sandwich (2004) .... Katherine
• "It's All Relative" .... Park Ranger (1 episode, 2004)
       - Who's Camping Now (2004) TV episode .... Park Ranger
• The Hazing (2004) .... Jill
  ... aka Dead Scared (USA: new title)
• "Less Than Perfect" .... Nurse Benson (1 episode, 2004)
       - 22 Minus 1 Equals 4 (2004) TV episode .... Nurse Benson
• "Monk" .... Reporter (1 episode, 2004)
       - Mr. Monk and the T.V. Star (2004) TV episode .... Reporter
• "Rock Me, Baby" .... Stacey (1 episode, 2003)
       - Prior Engagement (2003) TV episode .... Stacey
• "Wild On..." .... Hostess (1 episode, 2002)
       - Rio (2002) TV episode .... Hostess
• "That's Life" .... Reporter (1 episode, 2002)
       - All About Lydia (2002) TV episode .... Reporter